Part:BBa_J45199

Banana odor enzyme (ATF1) generator

BBa_J45199 takes as input a transcriptional signal (PoPS) and produce as output the ATF1 enzyme that catalyzes production of isoamyl acetate from isoamyl alcohol. Isoamyl acetate has a banana smell.

Usage and Biology

See BBa_J45014 for additional details.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 120
  Illegal BamHI site found at 1447
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 547
  Illegal SapI.rc site found at 1555
  

Functional Parameters: Austin_UTexas

BBa_J45199 parameters

Burden Imposed by this Part:

Burden Value: 2.2 ± 2.7%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.